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based on pulsed laser excitation and time-gated imaging detection 3D FLIM in conjunction with TriMScope
The major components of TauScope TD are:
FLIM images are generated by acquiring a series of time-gated fluorescence intensity images at a range of time delays after excitation and, for each pixel in the field of view, fitting the assumed decay profile using a nonlinear least squares fit. The major components of TauScope FD are:
FLIM images are generated by acquiring a series of phase shifted fluorescence intensity images at a range of phases and, for each pixel in the field of view, fitting the assumed decay profile using a nonlinear least squares fit. Functional information may be readily derived from fluorescence lifetime due to its dependence on fluorophore radiative and non-radiative decay rates. Imaging of fluorescence lifetime therefore provides a robust means of acquiring spatially resolved information regarding the local environment of a distributed fluorophore in biological tissue and other heterogeneous or turbid media. FLIM is independent of fluorescent probe concentration, photobleaching and intensity artefacts FLIM provides an additional contrast parameter and is very useful in multlabelling experiments FLIM provides information on molecular interations in the 1..10nm range FLIM is sensitive to molecular environment parameters such as pH, ion conc., viscosity, refractive index, Ca++, oxygen, polarity, FRET, electron transfer, conformation, ligand-receptor interactions, hydrophobic index... 3D FLIM The use of PicoStar camera in conjunction with the TriMScope allow generation of 3D FLIM data. PUBLICATIONS: Sensing cellular function and molecular activity in vivo using fluorescence lifetime imaging microscopy (FLIM) Video rate fluorescence lifetime imaging and fluorescence lifetime endoscopy Fluorescence lifetime imaging with picosecond resolution for biomedical applications Fluorescence lifetime three-dimensional microscopy with picosecond precision using a multifocal multiphoton microscope Fast excited state intramolecular proton transfer and subnanosecond dynamic Stokes shift of time-resolved fluorescence spectra of the 5-methoxysalicylic acid/diethyl ether complex. Time-resolved fluorescence of the bacteriophageT4 capsid protein gp23 Protein localization in living cells and tissues using FRET and FLIM High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging pH-dependent regulation of lysosomal calcium in macrophages Time-domain fluorescence lifetime imaging applied to biological tissue Noniterative Biexponential Fluorescence Lifetime Imaging in the Investigation of Cellular Metabolism by Means of NAD(P)H Autofluorescence Picosecond time-gated microscopy of UV-damaged plant tissue Fluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells Imaging fluorescence lifetime modulation of a ruthenium-based dye in living cells: the potential for oxygen sensing Autofluorescence Lifetime Imaging of Cultivated Cells Using a UV Picosecond Laser Diode Fluorescence lifetimes of molecular dye ensembles near interfaces |  | | |||||||
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time-doamin: 50ps lifetime resolution frequency domain: gain modulation up to 1GHz Homodyne imaging mode offers phase and modulation lifetime resolution down to 100ps